SESAME-HSQC

Examples on 13C/15N ubiquitin:

SESAME-HSQC: Full spectrum

Spin state selection for all spin multiplicities (in order NH, CH, CH₃, CH₂)

IS optimized sequence (pulse figure b) for NH and CαHα


I₂S optimized SESAME-HSQC for measuring geminal ²J_HH along with one-bond couplings:

Reference:
P. Würtz and P. Permi:
SESAME-HSQC for simultaneous measurement of NH and CH scalar and residual dipolar couplings.
Magn Reson Chem 2007; 45(4):289-95. [PUBMED]

Download and installation

Download complete package: sesamehsqc.tgz

Installation instructions:

SESAME-HSQC pulse sequences for Varian Unity Inova. Tested with Vnmr 6.1c.

Please read the disclaimer before using the sequence. For usage/processing refer to the manual page.

Installation setup

1. Login at the user account in which the installation should be carried out
2. Save the sesamehsqc.tgz file at a location with write permission for the user who is carrying out the installation.
3. Unpack the file with the command: tar xzvf sesamehsqc.tgz
4. Copy the files to the corresponding vnmrsys directories

Running and processing experiments

1. Start with an ¹⁵N HSQC experiment.
2. Load the parameter file.
3. Run the sesamehsqc macro for setting up the experiment.
4. Set relevant parameters such as pulse widths and power levels (specified in manual).
5. Adjust receiver gain and start experiment.
6. Standard processing by sesamehsqc_a or sesamehsqc_b for selecting individual lines in couplet doublet. For measuring geminal couplings use sesame_ipapx. Further instructions given in manual page.
7. ¹³C referecing by sesame_ch(freq) and ¹⁵N axis referecing by sesame_nh(freq).

For questions or requests please contact Dr. Perttu Permi, perttu.permihelsinki.fi