For ischemic brain injury we are focused on delayed events. Manipulations of these events can result in restoring damaged neuronal circuits. We are focused on neurotrophic factors, endoplasmic reticulum stress, and inflammation that occurs days after stroke.
In Parkinson's disease dopamine neurons, originating from substantia nigra, first lose their phenotype before they die. Our approach is to use novel neurotrophic factors to regenerate dopamine circuitry and slow down the progression of the disease.
We are using in vivo animal models to find novel drug targets in various cell types such as microglia, astrocytes and neurons. We use epifluorescence, luciferase and colorimetric -based reporter assays to find ligands for novel drug targets.