Di-Poi Group

    Contact:
    Nicolas Di-Poi
    Group Leader
    Tel: +358-50-4160551
    Fax: +358-29-4159930
    Email: nicolas.di-poi -at- helsinki.fi

    Institute of Biotechnology
    Research Program in Developmental Biology
    P.O. Box 65
    00014 University of Helsinki

    Street address:
    Viikinkaari 1, Biocenter 3
    00710 Helsinki

 

 

Histology Unit

The Histology Core Facility enable researchers to prepare tissue specimens for paraffin embedding, paraffin sectioning, plastic sectioning, cryosectioning, vibratome sectioning, and histological stains. It also provides technical assistance and training in histological techniques to investigators throughout Viikki and the University of Helsinki.

We are part of the HiLAPS platform, a HiLIFE research infrastructure with unique expertise in histotechnology, digital slide scanning, laboratory animal pathology, and tissue analysis (https://www.helsinki.fi/en/helsinki-institute-of-life-science/hilife-research-infrastructures).

The facility is equipped with 2 cryostat workstations (cryostat, microsope), 6 microtome workstations (microtome, microsope, water bath), 1 vibratome workstation (vibratome, microsope), 2 automated tissue processor machines for paraffin, 1 manual paraffin embedding station, 1 oven for manual paraffin processing, and 2 manual slide staining workstations.

The core is located at the Institute of Biotechnology, in rooms 5026 (tissue processing), 5032a (sectioning), and 5032b (histological staining) of Biocenter 2 building (Viikinkaari 5).

Facilities and Equipment

Tissue processing:
-Automated tissue processor Leica ASP300S
-Automated tissue processor Leica ASP200
-Manual paraffin embedding station Tissue-Tek

 

 

Tissue sectioning:
-Microtome Leica 2035 Jung Biocut
-Microtome Leica Jung RM 2055
-Microtomes Microm HM 355
-Microtomes Microm HM 355 S
-Vibratome Microm HM 650 V
-Cryostat Leica CM3050
-Cryostat Leitz 1720

 

Tissue deparaffinization/staining:
-Manual slide staining sets Tissue-Tek II

 

 

 

 

Equipment access
-All available equipments at the facility can be booked on site (online system coming soon).

-New users of the facility must participate to a hands-on training session (tissue processing & sectioning) before starting using the equipment. Please contact directly our technician Merja (merja.makinen@helsinki.fi) to arrange a time for a training and/or for services.

List of services and fees
Self-service:
-Automatic tissue processing (paraffin embedding station): 50€ / standard run
-Automatic tissue processing (paraffin embedding station): 75€ / long run
-Manual tissue processing (oven): 30€ / run
-Sectioning (microtome, cryostat, vibratome): 4€ / hour       
-Histological staining of sections: 5€ / use
-Deparaffinization: 4€ / use

Service by a technician:
-Paraffin-block sectioning: 35€ / hour                                                                   
-Hands-on training (tissue processing & sectioning): 35€ / hour
-Tissue processing (paraffin embedding station, first rack): 70€ / standard run
-Tissue processing (paraffin embedding station): 95€ / long run
-Automatic tissue processing (additional rack): 20€ / run

Fees include paraffin, standard disposable blades for sectioning, and chemicals for tissue processing, deparaffinization, and staining.

Contact:
Director: Dr Nicolas Di-Poï (nicolas.di-poi@helsinki.fi)
Technician: Merja Mäkinen (merja.makinen@helsinki.fi)

 


April 2014: Spring retreat of Developmental Biology Program (Tvärminne Biological station, seminar room)

April 2014: Spring retreat of Developmental Biology Program (Tvärminne Biological station, view from sauna)

May 2014: Construction of our farming box (Viikki campus)

May 2014: Plantation of tomatoes, carrots, zucchinis, radishes, flowers etc... (Viikki campus)

May 2014: Julia's birthday !

 

 

October-November 2014: Annual meeting of the Finnish Society for Developmental Biology (Hyytiälä Biological station, Halloween party)