Leica EM Pact High Pressure Freezing device
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Leica EM Pact High Pressure Freezing device
(Leica Microsystems)
Location: Room 1411.1
High Pressure Freezing (HPF) is the way to localise or characterise organelles,
subcellular components and gene products in EM
without the disadvantages of classical fixation methods:
- HPF gives better structural preservation and retains antigenicity
far better than conventional chemical fixation.
- HPF gives around 200 μm depth of good freezing with all
room pressure cryofixation techniques, (plunge, slam and jet
freezing) samples can only be vitrified to depths of 5-20 μm.
This is a ten-fold increase in the depth of amorphous ice!
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