FEI Quanta 250 field emission gun SEM
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Location: Room 1415
installed on FEI Quanta 250 FEG
Features:
- Unique quality of the alignment allowing a perfect 3D visualisation
- 3View allows automated acquisition of 3D ultrastructure by sequentially imaging a
freshly cut, resin-embedded block face by a high-precision ultramicrotome within the SEM chamber.
- Unlike the 3D imaging techniques in light microscopy, the spatial resolution
provided by 3View in the Z direction is comparable to its X-Y resolution and does not degrade with depth.
- The cut step size can be set from 20 to 200 nanometers.
- The images are acquired from the block-face, not the sections which are discarded.
Image capture is done using a backscatter electron detector.
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Gatan 3View cutting and imaging animation.
Gatan 3View cutting and imaging animation
Animation by Julia Kuhl for the Winfried Denk Lab |
Desktop
Smooth Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
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Endoplasmic reticulum organization in an early-metaphase
During cell division, cells become loosely attached to the substratum and, as a consequence, round up. The rounding up process affects ER network
organization in a way that it becomes packed into concentric layers. At the same time, the ER sheets undergo transition towards more fenestrated and tubular form.
Serial Block Face Scanning Electron Microscopy (SBF-SEM) dataset and animated model of an early-metaphase Huh-7 cell show a more spread endoplasmic reticulum (ER)
network organization on the more flat part of the cell whereas on the more rounded part the ER is packed into concentric layers.
The specimen was chemically fixed prior staining for SBF-SEM, and images were acquired at 4500x magnification providing a voxel size of 16 x 16 x 50 nm.
See more in Mol Biol Cell (2012) 23:13 2424-2432
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Modeling of a mitotic cell
A model of Huh-7 metaphase cell showing chromosomes, endoplasmic reticulum network, mitochondria and lipid bodies/lysosomes.
The model was segmented from a dataset obtained by Serial Block Face Scanning Electron Microscopy (SBF-SEM).
The specimen was chemically fixed prior staining for SBF-SEM, and images were acquired at 1000x magnification providing a voxel size of 27 x 27 x 50 nm.
See more in Mol Biol Cell (2012) 23:13 2424-2432
The dataset was provided by Joel Mancuso, Gatan Inc and processed, analized and
segmented in Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki.
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