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University of Helsinki Biocentrum Helsinki Genome Biology Unit
 

Genome Biology Unit (GBU)

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Contact information:

tel: 02-941 25581
bch-gbu@helsinki.fi

Kaisa Laajanen M Sc
Susanna Räsänen B Sc (pharm)
Tea Vallenius MD, PhD

 

High-Content Screening (HCS)

harri

Centre for Drug Research and GBU have joined forces to acquire a High Content Screening Microscope that will be operated through Light Microscope Unit.

 

Harri Jäälinoja
harri.jaalinoja@helsinki.fi

 

LMU hosts several instruments that can be used for High-Content Screening projects (for more details see HCS instruments at LMU http://www.biocenter.helsinki.fi/bi/lmu/instruments_hca.htm).

  1. Leica SP5II HCS-A
    - confocal microscope
    - motorized stage contolled by Matrix Screener software
    - suitable also for live cell work
    - can be set up for Computer Aided Microscopy (CAM)
  2. Thermo Scientific Cellomics CellInsight
    - desktop imaging system for well-plates
    - only for fixed samples
  3. Cell-IQ
    - continuous cell culturing platform
  4. 3i Marianas (see http://www.biocenter.helsinki.fi/bi/lmu/instruments_wf.htm)
    - advanced system for multiple imaging techniques
    - can be set up to image well-plates
     

True confocal HCA system, Leica TCS SP5 II HCS

Software

definiersThe CellInsight and Cell-IQ instruments have their own analysis software. For data collected with other instruments LMU can create a custom solution using both commercial (Imaris, Matlab) and open-source (Fiji, CellProfiler, KNIME) software.

 

Data handling

COLUMBUS

LMU can offer different solutions for data management (see LMU Image Storage and Analysis). At the moment, the largest resource available is a 40 TB quota in CSC's Ida system, but this will probably soon be complemented with more local storage from University of Helsinki IT.

 

 

Related Links

Biomedicum Imaging Unit provides with wide-field imaging (20x) with good stackers (robotic)Cellomics ArrayScan.