InBase is a curated database devoted to inteins.
When referencing InBase, please use the following InBase
Reference: Perler, F. B. (2002). InBase, the Intein Database. Nucleic
Acids Res. 30, 383-384.
What is Protein Splicing?
Protein splicing is defined as the excision of an intervening protein sequence (the INTEIN) from a protein precursor and the concomitant ligation of the flanking protein fragments (the EXTEINS) to form a mature extein host protein and the free intein (Perler 1994). Protein splicing results in a native peptide bond between the ligated exteins (Cooper 1993). Extein ligation differentiates protein splicing from other forms of autoproteolysis. Conserved intein motifs differentiate inteins from other types of in-frame sequences present in one homolog and absent in another homolog or from other types of protein rearrangements.
Please Note: The term 'Protein Splicing' has been associated with inteins since 1994 (Perler 1994). Recent papers have described protein rearrangements that are not intein-mediated. The mechanism of these rearrangements is currently unknown, but preliminary evidence suggests that they are mediated by various cellular enzymes. For clarity, we suggest calling these non-intein mediated events either protein rearrangements or Protein Editing.
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The Intein Registry Curator adds new information to intein records as submitted and attempts to fill in all fields if not provided by the contributor. The 'Initially Contributed by' field only indicates the person who made the initial submission or the name of the scientist associated with the intein if the submission is made by the curator. The 'Independently Found by' field indicates that another person has independently identified and submitted information about an intein prior to publication or release in the database. Updates are acknowledged in the 'Reference' and 'Comments' fields. Researchers should be aware that once an intein sequence is available in a public database, it may be identified and submitted by anyone searching databases for inteins. We therefore urge the initial discoverer to submit his or her entry as soon as possible to avoid seeing their intein submitted by another party. Entries may be submitted confidentially.
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