Mart Saarma Lab

Contact information

Mart Saarma
Professor 
Tel. +358 50 5002726
Fax. +358 2941 59366
e-mail: mart.saarma-at-helsinki dot fi

Institute of Biotechnology
P.O.Box. 56, 00014 University of Helsinki
Street address: Viikinkaari 5D, Biocenter 2

 

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Publications of Saarma lab members from 2015

ORIGINAL ARTICLES

  1. Domanskyi A, Saarma M, Airavaara M (2015) Prospects of Neurotrophic Factors for Parkinson's Disease: Comparison of Protein and Gene Therapy. Hum Gene Ther. 26(8):550-9. doi: 10.1089/hum.2015.065.
  2. Kemppainen S, Lindholm  P, Galli E, Lahtinen H-M, Koivisto H, Hämäläinen E, Saarma M & Tanila H (2015) Cerebral dopamine neurotrophic factor improves long-term memory in APP/PS1 transgenic mice modeling Alzheimer's disease as well as in wild-type mice. Behavioural Brain Research, May 11;291:1-11. doi: 10.1016/j.bbr.2015.05.002.
  3. opra, J, Vilenius, C, Grealish, S, Harma, M-A, Varendi, K, Lindholm, J, Castren, E, Voikar, V, Bjorklund, A, Piepponen, T. P, Saarma, M*, Andressoo, J-O*. (2015) GDNF is not required for catecholaminergic neuron survival in vivo. Nature Neuroscience 18 (3): 319-322. * Equal contribution.
  4. Kumar A, Kopra J, Varendi K, Porokuokka LL, Panhelainen A, Kuure S, Marshall P, Karalija N, Härma MA, Vilenius C, Lilleväli K, Tekko T, Mijatovic J, Pulkkinen N, Jakobson M, Jakobson M, Ola R, Palm E, Lindahl M, Strömberg I, Võikar V, Piepponen TP, Saarma M’, Andressoo JO’.(2015) GDNF Overexpression from the Native Locus Reveals its Role in the Nigrostriatal Dopaminergic System Function. PLoS Genet.  Dec 17;11(12):e1005710. doi: 10.1371/journal.pgen.1005710. ‘Equal contribution.
  5. Lindholm D, Mäkelä J, Di Liberto V,Mudò G, Belluardo N, Eriksson O and Saarma M. (2015) Current disease modifying approaches to treat Parkinson´s disease. CMLS, in press.
  6. Mizui T, Ishikawa Y, Kumanogoh H, Lume M, Matsumoto T, Hara T, Yamawaki S, Takahashi M, Shiosaka S, Itami C, Uegaki K, Saarma M, Kojima M (2015) BDNF pro-peptide actions facilitate hippocampal LTD and are altered by the common BDNF polymorphism Val66Met, PNAS 112(23):E3067-74
  7. Mutso M, Nikonov A, Pihlak A, Žusinaite E, Viru L, Selyutina A, Reintamm T, Kelve M, Saarma M, Karelson M, Merits A (2015) RNA Interference-Guided Targeting of Hepatitis C Virus Replication with Antisense Locked Nucleic Acid-Based Oligonucleotides Containing 8-oxo-dG Modifications. PLoS One. 2015 Jun 3;10(6):e0128686. doi: 10.1371/journal.pone.0128686. eCollection 2015.
  8. Voutilainen MH, Arumäe U, Airavaara M, Saarma M. (2015) Therapeutic potential of the endoplasmic reticulum located and secreted CDNF/MANF family of neurotrophic factors in Parkinson's disease. FEBS Lett. 589 (2015) 3739–3748.
  9. einheimer, I, Jiu, Y, Rajamaki, M-L, Matilainen, O, Kallijarvi, J, Cuellar, W J; Lu, R, Saarma, M, Holmberg, C I, Jantti, J, Valkonen, J P T (2015) Suppression of RNAi by dsRNA-Degrading RNaseIII Enzymes of Viruses in Animals and Plants. PLoS Pathogens.11 (3); e1004711 DOI:10.1371/journal.ppat.1004711

Publications of Saarma lab members from 2014

ORIGINAL ARTICLES

  1. Nadella R, Voutilainen MH, Saarma M, Gonzalez-Barrios JA, Leon-Chavez BA, Jiménez JM, Jiménez SH, Escobedo L, Martinez-Fong D (2014) Transient transfection of human CDNF gene reduces the 6-hydroxydopamine-induced neuroinflammation in the rat substantia nigra. J Neuroinflammation 11:209
  2. Ihermann A, Lume M, Miinalainen IJ, Pirttiniemi, A, Gui, Y, Peränen, J, Charron, J, Saarma, M, Costantini F & Kuure S. (2014) Mitogen-activated protein kinase (MAPK) pathway regulates branching by remodeling epithelial cell adhesion. PLoS Genetics, 10(3):e1004193. doi: 10.1371/journal.pgen.1004193. eCollection 2014
  3. Lindahl M, Danilova T, Palm E, Pulkkila P, Voikar V, Hakonen E, Ustinov J, Andressoo J-O, Harvery B, Otonkoski T, Rossi J and Saarma M. (2014). MANF is indispensable for the proliferation and survival of pancreatic β-cells. Cell Reports 7(2):366-375
  4. Varendi K, Airavaara A, Anttila J, Vose S, Planken A, Saarma M, Mitchell JR, Andressoo J-O. (2014) PLOS ONE and entitled "Short-term Preoperative Dietary Restriction is Neuroprotective in a Rat Focal Stroke Model, 9(4):e93911. doi: 10.1371


Publications of Saarma lab members from 2013

ORIGINAL ARTICLES

  1. Glerup S, Lume M, Olsen D, Nyengaard JR, Vaegter, CB, Gustafsen C, Christensen EI, Kjolby M, Hay-Schmidt A, Lande AD, Madsen P, Saarma M, Nykjaer A and Petersen CM. (2013) SorLA controls neurotrophic activity through sorting of GDNF and its receptors GFRα1 and RET. Cell Reports 3(1):186-99.
  2. Bäck S, Peränen J, Galli E, Pulkkila P, Lonka-Nevalaita L, Tamminen T, Voutilainen MH, Raasmaja A, Saarma M, Männistö PT, Tuominen RK. (2013) Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson's disease. Brain Behav. 3(2):75-88.
  3. Piccinini E, Kalkkinen N, Saarma M, Runeberg-Roos P. (2013) Glial cell line-derived neurotrophic factor: characterization of mammalian posttranslational modifications. Ann Med. 45(1):66-73.
  4. Lindström R, Lindholm P, Kallijärvi J, Yu LY, Piepponen TP, Arumäe U, Saarma M, Heino TI. Characterization of the structural and functional determinants of MANF/CDNF in Drosophila in vivo model. (2013) PLoS One. (2013) 8(9):e73928. doi: 10.1371/journal.pone.0073928.
  5. Yu SJ, Tseng KY, Shen H, Harvey BK, Airavaara M, Wang Y (2013) Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats. PLoS One. 2013 Dec 2;8(12):e81750
  6. Henderson MJ, Richie CT, Airavaara M, Wang Y, Harvey BK (2013) Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors. J Biol Chem. 288(6):4209-25
  7. Ichim G, Genevois AL, Ménard M, Yu LY, Coelho-Aguiar JM, Llambi F, Jarrosson-Wuilleme L, Lefebvre J, Tulasne D, Dupin E, Le Douarin N, Arumäe U, Tauszig-Delamasure S, Mehlen P (2013) The dependence receptor TrkC triggers mitochondria-dependent apoptosis upon Cobra-1 recruitment. Mol Cell 51:632-46
  8. Jakobson M, Jakobson M, Llano O, Palgi J, Arumäe U (2013) Multiple mechanisms repress N-Bak mRNA translation in the healthy and apoptotic neurons. Cell Death Dis, 4: e777
  9. Heermann S, Mätlik K, Hinz U, Fey J, Arumäe U, Krieglstein K (2013) GDNF mediates survival of murine sympathetic precursors. J Neurosci Res., 91:780-5

OTHER PUBLICATIONS

  1. Saarma M, Castrén E. (2013) Lahkus närvikasvufaktori avastaja Rita Levi-Montalcini. Sirp 2 (3424. 11.01.2013.


Publications of Saarma lab members from 2012

ORIGINAL ARTICLES

  1. Airavaara M., Harvey B. K., Voutilainen M. H., Shen H., Chou J., Lindholm P., Lindahl M., Tuominen R. K., Saarma M., Wang Y., Hoffer B. (2011). CDNF protects the nigrostriatal dopamine system and promotes recovery after MPTP treatment in mice. Cell Transplantation, 21, 1213–1223.
  2. Glerup S*, Lume M*, Olsen D, Nyengaard JR, Vaegter, CB, Gustafsen C, Christensen EI, Kjolby M, Hay-Schmidt A, Lande AD, Madsen P, Saarma M, Nykjaer A and Petersen CM. (2013) SorLA controls neurotrophic activity through sorting of GDNF and its receptors GFRα1 and RET. Cell Reports, 3, 1-14. *shared first authors.
  3. Feng S, Knödler A, Ren J, Zhang J, Zhang X, Hong Y, Huang S, Peränen J, Guo W.A. (2012). Rab8 guanine nucleotide exchange factor-effector interaction network regulates primary ciliogenesis. J Biol Chem.  287(19):15602-15609.
  4. Jakobson M, Lintulahti A, Arumäe U (2012) mRNA for N-Bak, a neuron-specific BH3-only splice isoform of Bak, escapes nonsense-mediated decay and is translationally repressed in the neurons. Cell Death Dis, 3:e269
  5. Kallijärvi J, Stratoulias V, Virtanen K, Hietakangas V,  Heino TI, and Saarma M. (2012)Characterization of Drosophila GDNF receptor-like and evidence for its evolutionarily conserved interaction with Neural Cell Adhesion Molecule (NCAM)/FasII. PLoS ONE 7(12): e51997. doi:10.1371/journal.pone.0051997
  6. Kysenius K, Muggalla P, Mätlik K, Arumäe U, Huttunen H (2012) PCSK9 regulates neuronal apoptosis by adjusting apoer2 levels and signaling. Cell. Mol. Life Sci., 69:1903-16.
  7. Pitkonen M, Hippeläinen E, Raki M, Andressoo JO, Urtti A, Männistö PT, Savolainen S, Saarma M, Bergström K. (2012) Advanced brain dopamine transporter imaging in mice using small-animal SPECT/CT. EJNMMI Res. 2(1):55. doi: 10.1186/2191-219X-2-55.
  8. Shulga A, Magalhaes A, Autio H, Plantman S, Di Lieto A, Nykjaer A, Carlstedt T, Risling M, Arumäe U, Castren E, Rivera C. (2012) The loop diuretic bumetanide blocks post-traumatic p75NTR upregulation and rescues injured neurons. J. Neurosci., 32:1757-1770.
  9. van de Ven M, Andressoo JO, van der Horst GT, Hoeijmakers JH, Mitchell JR. (2012) DNA Repair (Amst) 11:874-83
  10. Wang Y, Chou J, Yu SJ, Airavaara M, Shen H, Harvey BK (2012) Suppression of endogenous PPARγ increases vulnerability to methamphetamine –induced injury in mouse nigrostriatal dopaminergic pathway. Psychopharmacology 221:479-92.
  11. Weber-Boyvat M, Zhao H, Aro N, Yuan Q, Chernov K, Peränen J, Lappalainen P, Jäntti J.(2012). A conserved regulatory mode in exocytic membrane fusion revealed by Mso1p membrane interactions. Mol Biol Cell. 2012 Nov 28. [Epub ahead of print]

 

REVIEWS AND BOOK CHAPTERS

  1. Airavaara M*, Voutilainen MH*, Wang Y, Hoffer B (2012) Neurorestoration.  Invited review Parkinsonism & Related Disorders. * equal contribution 18 Suppl 1:S143-6
  2. Kuure, S. Analysis of migration in primary ureteric bud epithelial cells. Book chapter in Methods in molecular biology, Kidney Development: Methods and Protocols. vol. 886: 147-55.  Ed. Jon Walker & Odysse Michos.
  3. Saarma M, Lindholm P. Arumäe U. (2012) Neurotrophic Factors. Chapter 7. Movement Disorders, 3rd Edition, edited by Watts, Standaert and Obeso, 129-140.

 

OTHER PUBLICATIONS

  1. Eero Castrén, Mart Saarma, Dan Lindholm Tutkija löysi uutta tietoa hermostosta. Professori Hans Thoenen. Helsingin Sanomat 13.07. 2012.
  2. Saarma, M: Teaduse kvaliteedist ja innovatsioonist. Riigikogu Toimetised 25, 9-20, 2012

 

RECENT PATENTS

1. Saarma M, Karelson M, Pilv M, Bespalov MM. Methods of facilitating neural cell survival using GDNF family ligand (GFL) mimetics or RET signalling pathway activators European Patent No 10790954.1-2101, 2012

2. Penn R, Runeberg-Roos P, Bespalov MM, Saarma M. Neurturin molecules, US Patent 8,445,432. May 21, 2013

3. Saarma M, Lindholm P, Voutilainen M, Peranen J, Tuominen R, Airavaara M, Leppanen V-M, Lindahl M, Andressoo JO (2014). Neurotrophic factor MANF and uses thereof. US Patent 8,853,166.  October 7, 2014

4. Saarma M, Karelson M, Bespalov M, Pilv M. Methods facilitating neuronal survival using gdnf family ligand (gfl) mimetics or ret signaling pathway activators. US Patent No 8,901,129, December 2, 2014

5. Nevalaita L, Saarma M. Splice variants of GDNF and uses thereof. European Patent, positive decision to 12 189 585.8-1456, April 20, 2015


SELECTED PUBLICATION FROM PREVIOUS YEARS

  • Lindahl M., Poteryaev D, Yu L, Arumäe U, Timmusk T, Bongarzone I, Aiello A, Pierotti M A, Airaksinen MS, Saarma M. (2001). Human GFRa4 is the receptor for persephin, and is selectively expressed in normal and malignant thyroid medullary cells. J Biol Chem, 276 (12), 9344-9351. Article
  • Airaksinen MS, Saarma M (2002) The GDNF family: signalling, biological functions and therapeutic value. Nat Rev Neurosci 3: 383-394. Article
  • Rivera C, Li H, Thomas-Crusells J, Lahtinen H, Viitanen T, Nanobashvili A, Kokaia Z, Airaksinen MS, Voipio J, Kaila K, Saarma M (2002) BDNF-induced TrkB activation down-regulates the K+-Cl- cotransporter KCC2 and impairs neuronal Cl- extrusion. J Cell Biol 159: 747-752. Article
  • Yu L- Y,  Jokitalo E, Sun F-S, Mehlen P, Lindholm D, Saarma M  and Arumäe U.  (2003)  GDNF-deprived sympathetic neurons die via a novel nonmitochondrial pathway. J Cell Biol, 163: 987-997. Article
  • Popsueva A, Poteryaev D, Arighi E, Meng X, Angers-Loustau A, Kaplan D, Saarma M,  Sariola H. (2003). GDNF promotes tubulogenesis of GFRα1-expressing MDCK cells by Src-mediated phosphorylation of MET receptor tyrosine kinase. J Cell Biol, 161(1):119-129. Article
  • Leppänen VM, Bespalov MM, Runeberg-Roos P, Puurand U, Merits A, Saarma M, Goldman A (2004) The structure of GFRalpha1 domain 3 reveals new insights into GDNF binding and RET activation. EMBO J 23: 1452-1462. Article
  • Rivera C, Voipio J, Thomas-Crusells J, Li H, Emri Z, Sipilä S, Payne J A, Minichiello L, Saarma M and Kaila K. (2004) Mechanism of activity-dependent downregulation of the neuron-specific K-Cl cotransporter KCC2. J Neurosci, 24(19):4683-4691. Article
  • Hiltunen JO, Laurikainen A, Klinge E, Saarma M. (2005) Neurotrophin-3 is a target-derived neurotrophic factor for penile erection-inducing neurons. Neuroscience 133(1): 51-58. Article
  • Bespalov, M., Saarma, M. (2007) GDNF receptor complex is an emerging drug target. Trends in Pharmacological Sciences 28 (2), 68-74. Article
  • Mijatovic J, Airavaara M, Planken A, Auvinen P, Raasmaja A, Piepponen TP, Costantini F, Ahtee L, Saarma M (2007) Constitutive Ret activity in knock-in multiple endocrine neoplasia type B mice induces profound elevation of brain dopamine concentration via enhanced synthesis and increases the number of TH-positive cells in the substantia nigra. J Neurosci 27: 4799-4809. Article
  • Yang J, Runeberg-Roos P, Leppänen V-M and Saarma M. (2007) The mouse soluble GFRα4 receptor activates RET independently of its ligand persephin. Oncogene, 26 (26), 3892-3898. Article
  • Runeberg-Roos P, Virtanen H, Saarma M (2007) RET(MEN 2B) is active in the endoplasmic reticulum before reaching the cell surface. Oncogene 26: 7909-7915. Article
  • Li H, Khirug S, Cai C, Ludwig A, Blaesse P, Kolikova J, Afzalov R, Coleman S K, Lauri S, Airaksinen M S, Keinänen K, Khiroug L, Saarma M, Kaila K and Rivera C. (2007) KCC2 interacts with the dendritic cytoskeleton to promote spine development. Neuron 56(6), 1019-1033. Article
  • Lindholm P, Voutilainen MH, Lauren J, Peranen J, Leppänen VM, Andressoo JO, Lindahl M, Janhunen S, Kalkkinen N, Timmusk T, Tuominen RK, Saarma M (2007) Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo. Nature 448: 73-77. Article
  • Parkash V, Leppanen VM, Virtanen H, Jurvansuu JM, Bespalov MM, Sidorova YA, Runeberg-Roos P, Saarma M, Goldman A (2008) The structure of the glial cell line-derived neurotrophic factor-coreceptor complex: insights into RET signaling and heparin binding. J Biol Chem 283: 35164-35172. Article
  • Lindholm P, Peränen J, Andressoo JO, Kalkkinen N, Kokaia Z, Lindvall O, Timmusk T and Saarma M. (2008) MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain. Mol Cell Neurosci, 39(3), 356-371. Article
  • Katritzky A R, Dobchev D A, Stoyanova-Slavova I B, Kuanar M, Bespalov M M, Karelson M and Saarma M. (2008) Novel computational models for predicting dopamine interactions. Exp Neurol, 211 (1), 150-171. Article
  • Andressoo J-O and Saarma M. (2008) Signalling mechanisms underlying development and maintenance of dopamine neurons. Curr Opin Neurobiol, 18, 297–306. Article
  • Palgi M, Lindström R, Peränen J, Piepponen TP, Saarma M, Heino TI (2009) Evidence that DmMANF is an invertebrate neurotrophic factor supporting dopaminergic neurons. Proc Natl Acad Sci USA 106: 2429-2434. Article
  • Voutilainen MH, Bäck S, Porsti E, Toppinen L, Lindgren L, Lindholm P, Peränen J, Saarma M*, Tuominen RK (2009) Mesencephalic astrocyte-derived neurotrophic factor is neurorestorative in rat model of parkinson's disease. J Neurosci 29, 9651-9659 (* corresponding author). Article
  • Lonka-Nevalaita L, Lume M, Leppänen S, Jokitalo E, Peränen J and Saarma M. (2010) Characterization of the intracellular localization, processing and secretion of two GDNF splice isoforms. J. Neurosci. , 30(34):11403-11413. Article
  • Bespalov MM, Sidorova Y A, Tumova S, Ahonen-Bishopp A, Magalhães AC, Kulesskiy E, Paveliev M, Rivera C, Rauvala H, and Saarma M. (2011) Heparan sulfate proteoglycan syndecan-3 is a novel receptor for GDNF, neurturin and artemin. J. Cell Biol., 192 (1) 153-169. Article
  • Hellman M, Arumäe U, Yu LY, Lindholm P, Peränen J, Saarma M*, Permi P* (2011) Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) Has a Unique Mechanism to Rescue Apoptotic Neurons. J Biol Chem 286: 2675-2680. * Equal contribution. Article
  • Voutilainen MH, Bäck S, Peränen J, Lindholm P, Raasmaja A, Männistö PT, Saarma M, Tuominen RK. (2011). Chronic infusion of CDNF prevents 6-OHDA-induced deficits in a rat model of Parkinson's disease. Exp Neurol. 228(1): 99-108. Article